High efficiency electroporation of intactCorynebacterium glutamicumcells
نویسندگان
چکیده
منابع مشابه
High efficiency transformation of E. coli by high voltage electroporation
E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The process is highly dependent on two characteristics of the electrical pulse: the ele...
متن کاملHigh-efficiency transformation of Rhizobium leguminosarum by electroporation.
Electrotransformation of Rhizobium leguminosarum was successfully carried out with a 15.1-kb plasmid, pMP154 (Cmr), containing a nodABC-lacZ fusion by electroporation. The maximum transformation efficiency, 10(8) transformants/microg of DNA, was achieved at a field strength of 14 kV/cm with a pulse of 7.3 ms (186 Omega). The number of transformants was found to increase with increasing cell den...
متن کاملHigh-efficiency transformation of Chlamydomonas reinhardtii by electroporation.
We have established a high-efficiency method for transforming the unicellular, green alga Chlamydomonas reinhardtii by electroporation. Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid of argininosuccinate lyase (encoded by ARG7), in the presence of the plasmid pJD67 (which contains ARG7) was used to optimize conditions for the introduction of exogenous DNA. The condit...
متن کاملImproved efficiency M13 cloning using electroporation.
We compared the efficiency of transformation of recombinant M13 DNA obtained using the traditional calcium chloride method with that obtained by electroporatlon. M13 cloning experiments are often hampered by low yields I.e. insufficient numbers of recombinant plaques are generated. Low concentration of the target DNA, poor ligation due to target DNA impurity and small target fragment size are c...
متن کاملD-Glucosamine Promotes Transfection Efficiency during Electroporation
D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1989
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1989.tb03677.x